|
|
Analysis of chromosomal aberrations in sperm by fluorescence in situ hybridization
Bendová, Petra ; Diblík, Jan (advisor) ; Novotná, Drahuše (referee)
The presented bachelor work is focused on the determination of frequency chromosomally abnormal sperm in the semen of healthy men (donors) with normal karyotype (46, XY). The important process, which plays an irreplaceable role in the development of numerical aberrations of chromosomes or structural abnormalities in the segregation of the gametes, is meiosis. Therefore, I devote much attention on meiosis in the theoretical part. The theoretical part is focused on the process of pre mature sperm (spermatogenesis), and the consequences of fertilize the oocyte by aneuploid sperm. In my work I present an overview of numerical abnormalities in autosomes and gonosomes and their frequency and distribution of gametes in healthy men. I also focused on the distribution and a brief description of structural aberrations affecting chromosomes and not least I paid attention on method of multicolor interphase fluorescence in situ hybridization, which in combination with sperm chromatin dekondenzation become irreplaceable and valuable research tool for rapid analysis of chromosomal abnormalities in large sperm samples. The experimental part of bachelor work deals with monitoring the frequency of selected numerical abnormalities in sperm samples of five donors aged 23 to 30 years with the use of I-FISH (fluorescence in situ...
|
|
| |
|
|
The "Bodies" Probe into the Thinking of the Czech Society
Dobošová, Andrea ; Matějek, Jaromír (advisor) ; Ovečka, Libor (referee)
Having been on for ten years, "Bodies" The Exhibition still arouses strong controversial reactions. The authors of the exhibition of dead bodies speak about education, whereas the opponents object it is an unethical business. The Czech society has its system of values, which has developed over centuries. The objective of my Bachelor thesis is to monitor the social affairs, which reflect the change of mentality and orientation of values as influenced by new possibilities of self- fulfilment. In media, and not only there, a sheer variety of opinions was given on the exhibition. Leading Czech scholars, including medical doctors, theologians, journalists and art historians, gave their statements; their views being mostly a rejection. Judging from the number of tickets sold, the interest of the public to see the exhibition is great. The purpose of my thesis is to record a probe by means of the analytic method, within which I describe the Czech society in a specific conduct perceived by the majority as non-problematic. The current situation is an excellent way to learn about the real world we live in.
|
|
| |
|
|
NK cells and KIR receptors: their importance in haematopoietic stem cell transplantations in leukemia
Ledvinková, Anna ; Vraná, Milena (advisor) ; Pačes, Jan (referee)
NK cells and KIR receptors: their importance in haematopoietic stem cell transplantations in leukemia Abstract This bachelor thesis discusses the importance of NK (natural-killer) cells in leukemias. It focuses on the structure and reactivity of NK cells, and especially on transmembrane KIR receptors (killer cell immunoglobulin-like receptors) of NK cells, that play an important role in the elimination of leukemic cells (graft-versus leukemia reaction, GvL) and thus in the overall prognosis of the disease. Activation and inhibitory receptors of KIR, by their cooperation, control the cytotoxic activity of NK cells. Thus, the typing of KIR receptor genes in hematopoietic stem cell donors can predict treatment success. KIR genes examination is mainly used in patients diagnosed with acute myeloid leukemia. Keywords: KIR receptor, NK cells, haematopoietic stem cell transplantation, HSCT, leukemia, donor, recipient
|
|
|
Human milk bank and their reason
PODLAHOVÁ, Pavla
This bachelor thesis is dedicated to banks of human milk and their purpose. The paper is divided into theoretical part, which describes the lactation's development, the composition of breast milk, techniques and advantages of breast feeding and a practical part. History of human milk banks, their function and utilization are also included. The practical part of this thesis consists of qualitative and quantitative research. Two research goals were set up - to get to know about specifics of work of the staff in human milk banks and to map the mothers' awareness of the possibility of human milk banks utilization after childbirth. In terms of the goal one, a research question was set: VO1: What are the specifics of work of the staff in human milk banks? In terms of the second goal, the hypothesis was set: H1: Are there any differences in awareness within mothers of babies who are born in term versus those who are born before the term of the possibility to utilize human milk banks? The qualitative research was done through questioning, in-depth interview method. The research sample was made up from the staff working in human milk banks, the interview was done with 4 workers. The interviews were transcribed and analysed thanks to "pen - paper" method. They were further divided into three categories and fifteen subcategories. The research question was set up: What are the specifics of work of the staff in human milk banks? The outcomes of the research showed that the staff in human milk banks are mostly working with the donors of breast milk, which is further being examined, pasteurized and frozen. The quantitative part was made through questionnaire in social media with the total number of 1000 respondents. The outcomes of the research were graphically represented and added by verbal comments. Hypothesis verification was done thanks to chi square test. The zero hypothesis was tested against the alternative hypothesis with the level of significance of 5 %. Afterwards the level of significance was compared with the final value from chi square test. The hypothesis H1 ended up with the result of < 0,1 %. This result means that the mothers of babies who are born before the term have bigger awareness about human milk banks. 91,2 % of respondents are sufficiently informed about the human milk banks. This bachelor thesis can be used as an informative source to women, students of midwifery as well as the source of information for various conferences.
|
|
|
Involvement of donors of blood plasma to the donor's registry bone marrow
Míková, Iveta ; Kordulová, Pavla (advisor) ; Chvojková, Kateřina (referee)
The Bachelor thesis is focusing on the possibility to exploit the potential of regular blood plasma donors for inclusion into the register of bone marrow donors. Based on the assumption, that this is the almost identical population and that the criteria for blood plasma donating are similar to the criteria for bone marrow donation, plasma donors are tested periodically on the infectious diseases transmitted through blood, they have experience with the intravenous collection and plasma donors are largely complying with the age limit needed for bone marrow register. The theoretical part describes anatomy and physiology of blood plasma and bone marrow and current knowledge on the issue for donating of blood plasma and bone marrow. Describes types and indications of bone marrow transplantation, registers of blood plasma and bone marrow donors, the motivation for donating, reliefs and remunerations for donors. I have used for the making of this thesis processed research from Bibliographia medica tschechoslovacia in the National medical library, personal source of reference literature, professional consultation, own experiences and another Bachelor thesis. Methods: The Thesis was prepared based on quantitative research in the form of none standard anonymous questionnaire. The target group were the donors...
|
|
|
The issue of collection and bone marrow transplantation in pediatric and adult patients
KONEČNÁ, Martina
This thesis aims to provide a closer look at the issue and presentation of a clear summary of information on the issue of bone marrow transplantation via the Review Method and Information Synthesis. The thesis contains a theoretical description of nurse intervention before and after extracting bone marrow from a voluntary donor, as well as a description of nurse´s interventions before and after transplantingextracted hematopoietic cells from a patient suffering from the indicating blood illness. Treating hemato-oncological diseases is a lengthy and very demanding process. It relies in transplanting hematopoietic cells, and it means a great hope to cure the ill person. The transplantation itself is preceded by a long row of examinations, medical and administrative procedures needed to ensure the highest hope of curing the ill patient possible. In the Czech Republic, there are two donor registers cooperating together, based on the principle of all-register international cooperation. Since the moment of graft conversion, the transplanted patient is in a highly risky state. The patient´s living days start to be counted again. Taking care of hematopoietic cell recipients, the patient´s maximal well-being, safety, psychological well-being and most importantly, avoiding possible complications which may significantly slow down or even destroy medical staff´s and the voluntary donor´s efforts to save another person´s life completely, are all necessary focal points of the process. The thesis describes specific procedures of care for patients suffering from different kinds of complications, with regard to the nurses´ work. Because there is a difference in caring and communicating with adult and child patients, one of the subchapters is devoted especially to caring for child oncological patients, and the options of adjusting the communication to their needs and their parents´ needs. To return the patient back to their best life quality possible, it is necessary to follow principles of a healthy lifestyle and correct nutrition principles.
|
|
|
The "Bodies" Probe into the Thinking of the Czech Society
Dobošová, Andrea ; Matějek, Jaromír (advisor) ; Ovečka, Libor (referee)
Having been on for ten years, "Bodies" The Exhibition still arouses strong controversial reactions. The authors of the exhibition of dead bodies speak about education, whereas the opponents object it is an unethical business. The Czech society has its system of values, which has developed over centuries. The objective of my Bachelor thesis is to monitor the social affairs, which reflect the change of mentality and orientation of values as influenced by new possibilities of self- fulfilment. In media, and not only there, a sheer variety of opinions was given on the exhibition. Leading Czech scholars, including medical doctors, theologians, journalists and art historians, gave their statements; their views being mostly a rejection. Judging from the number of tickets sold, the interest of the public to see the exhibition is great. The purpose of my thesis is to record a probe by means of the analytic method, within which I describe the Czech society in a specific conduct perceived by the majority as non-problematic. The current situation is an excellent way to learn about the real world we live in.
|
|
|
Kryoprezervace a transplantace spermatogonií kapra obecného
FUČÍKOVÁ, Michaela
Cryopreservation and transplantation of germ cells in fish provides a suitable tool for preserving genetic information. By method of surrogate reproduction, the offspring with characters of the chosen donor can be obtained. In this case of our commercially important species common carp. However, for the successful cryopreservation of the germ cells, a suitable protocol for each species must be established. Several cryoprotectants were tested. The best of them, Me2SO, regarding the viability of spermatogonia, was tested for its different concentrations depending also on the rate of freezing. Further testing, related to the effect of tissue size, incubation time and added sugar, was performed. The result of the assay identified best cryomedium composed of 2.5M dimethylsulfoxide, added sugar of 0.3M glucose, 1.5% BSA and 25nM Hepes dissolved in PBS. The most suitable size of tissue was 100 mg, incubation time was 30 min and coolig rate was -1 ° C/min. This protocol ensures the highest viability rate of cryopreserved spermatogonia of common carp. The second part of the work was to verify the success of the transplantation of cryopreserved and fresh spermatogonia into a suitably chosen recipient, the goldfish, which shares similar reproductive characteristics with carp, but also offers reduction of space requirements or resistance to koi-herpes virus. The transplanted germ cells colonized the germ line and started gametogenesis in 42.5% (cryopreserved spermatogonia) and 52.5% (fresh spermatogonia) goldfish recipients, which demonstrated that the transplantation of cryopreserved spermatogonia of common carp can be successfully achieved.
|
guest ::
